分类: 生物医药
细胞疗法
Cell therapy
#Global Cell Therapy R&D Pipelines Development

#China Cell Therapy Companies Layout
1,Approved by NMPA:
- Jun 2021 FosunKite(复星凯特)CD19 200K USD;
- Sep 2021 JW Therapeutics(药明巨诺)CD19 200K USD
2,Aurora/NL Users:
- FosunKite(复星凯特)
- Unicar-therapy(优卡迪)
- ImmunoChina(艺秒神州)
- GracellBio (亘喜)
- Xiangxue Pharmaceutical(香雪制药)
- LegendBiotech(传奇生物)
#Cell Therapy Market Trends
- Car-NK:B-ALL, Breast Cancer, HCC
- TIL :Melanoma, Cervical cancer
- TCR-T: Melanoma, sarcoma
- γδ T
- Crisp- Car-T
- Car- Treg

#Car-NK Potential Advantages
1,Better Safety
No GVHD and low CRS
2,Keep natural anti-tumor cell toxicity
NKp46、NKp44 and NKp30
3,“universal” product potential
From PBMC, UCB, HESC, IPSC

#Car-NK Targets in Hematological and Solid Tumors


#TCR-T for Solid Tumors

#TCR-T develop rapidly in 2021

#TIL Advantages in Solid Tumors
- Good tumor invasion
- Targeted non-selective, tumor-specific killer T cells
- Specific recognition of tumor antigens only
- No genetic engineering, cost saving

#Flow application in cell therapy
- Immune therapy biomarkers screening and evaluation
- Cell therapy products evaluation
- Patients immune function determination
- Monitoring MRD
#Immune therapy biomarkers screening and evaluation

#Cell therapy products evaluation
- Evaluation of transfection efficiency
- Knockout efficiency evaluation
- Tumor killing effectiveness evaluation
#Patients immune function determination
- Immune function database
- TBNK population
- T cell subsets —— dynamic observation
#Guiding Opinions for Clinical Trials of Cellular Immunotherapy Products (Trial)
Test Items | Platform | |
PK | VCN, Virus Vector Copy Number | qPCR/dPCR |
Car+ cell number | FCM | |
PD | Cytokine | ELISA/FCM |
Immunogenicity | Drug resistance antibody, ADA/HAMA, Anti-Drug Antibody | ELISA/FCM |
CTL, Cytotoxic T- Lymphocyte | FCM | |
Safety | RCL/RCR Replication Competent Lentivirus | qPCR |
Virus integration site detection | NGS | |
Lentiviral p24 protein detection | ELISA | |
Car-T cell phenotyping | Lymphocyte subsets, TSCM | FCM |
中国单细胞技术初创公司简介










Cytek DxP Athena 流式应用
调节性T淋巴细胞流式检测
细胞周期流式检测
【实验结果】
采用FlowJo软件自带的Cell Cycle工具进行分析,其中G1 mean:61979.77,G2 mean:124155.05,G2:G1=2.003。
细胞凋亡流式检测

细胞因子流式检测
6. 加入适量TNF-α-FITC,INF-γ-PE,按照试剂盒说明进行孵育;
7. PBS洗涤,去上清,PBS重悬后上机检测。
【实验结果】
双阳性区域(Q2、Q6)为CD3+T细胞细胞因子分布。
γδT细胞流式检测
6. 洗涤:PBS洗涤2次并重悬,流式上机检测。
【实验结果】
下图Q1单阴性为TCRγδT细胞。
NK细胞流式检测
间充质干细胞(MSC)流式检测
【实验结果】
Tube5为空白对照管、Tube1/3为单染管、Tube6/8为同型对照管、Tube7/9为多色样本管。显示,样本几乎不存在非特异性吸附,CD90和CD105阳性较强,其他未列出指标也都符合hMSC检测指标。